Effect of Simulated Microgravity on Mieralization of Human Dental Pulp Stem Cells on PLGA Scaffolds in Vivo.

Abstract

Abstract: Objective: To investigate the mineralized effect of simulated microgravity on human dental pulp stem cells(hDPSCs) on PLGA(poly lactic-co-glycolic acid) after subcutaneous transplantation into nude mice. Methods: hDPSCs were seeded into PLGA scaffolds and cultured for 72 hours, then cell-scaffold composites were randomly divided into 2 groups: normal gravity group and simulated microgravity group, which were both cultured in osteogenic induction medium. After 1 week, cell-scaffold composites of both groups were respectively transplanted into the subcutaneous tissue of immunodeficiency mice. The samples were retrieved after 4 weeks of implantation for histological(HE and Vonkossa) and immunohistochemical (DSPP) examinations. Results: Angiogenesis was visualized in HE stained sections. Vonkossa staining were positive for assessing mineral deposition. The expression level of DSPP in simulated microgravity group was significant higher than that in normal gravity group(P<0.05). Conclusion: Simulated microgravity is advantageous for the mineralization of hDPSCs in nude mice.

Key words: Human dental pulp stem cells , Rotary cell culture system、 Scaffold, Mieralization

CLC Number:

R780.1

XU Dan-dan, LI Yan-ping, PAN Shuang, et al.. Effect of Simulated Microgravity on Mieralization of Human Dental Pulp Stem Cells on PLGA Scaffolds in Vivo.[J]. Journal of Oral Science Research, 2015, 31(3): 234-236.

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