Effects of AhR Overexpression on the Porphyromonas gingivalis Lipopolysaccharide- caused Inflammatory Response of Macrophages under High Glucose Conditions.

doi:10.13701/j.cnki.kqyxyj.2018.12.016

Abstract

Abstract: Objective: To investigate the effects of enhanced expression of aryl hydrocarbon receptor (AhR) on the inflammatory response of macrophages induced by Porphyromonas gingivalis lipopolysaccharide (Pg LPS) under high glucose conditions.Methods: RAW264.7 murine macrophages were inoculated in the medium with high glucose and were transfected with recombinant plasmids to enhance AhR expression.Then, Pg LPS was added to the medium with high glucose to induce inflammatory response.Western blotting was chosen to examine the expression of AhR, Notch1, Jagged1 and iNOS in macrophages, and ELISA was used to detect the concentration of TNFα and IL1β in the culture supernatant.Results: Compared with the cells without enhanced AhR expression, the cells overexpressing AhR exhibited less expression of Notch1, Jagged1, iNOS, TNFα, and IL1β under the condition of Pg LPS stimulation superimposed on high glucose (P<0.05).Conclusion: Increased AhR expression can inhibit the inflammatory response of macrophages induced by Pg LPS under high glucose conditions, and the suppresive effect may be associated with the inhibition of Notch signaling by AhR.

Key words: Aryl hydrocarbon receptor, Porphyromonas gingivalis, Macrophage, Inflammatory responseHigh glucose

ZHONG Xing-hua, LI Hao, LI Wei. Effects of AhR Overexpression on the Porphyromonas gingivalis Lipopolysaccharide- caused Inflammatory Response of Macrophages under High Glucose Conditions.[J]. Journal of Oral Science Research, 2018, 34(12): 1329-1332.

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