Preparation and Characterization of Hollow Hydroxyapatite Microspheres Loaded with Simvastatin as Pulp Capping Agent.

doi:10.13701/j.cnki.kqyxyj.2016.07.011

Abstract

Abstract: Objective: To synthesize hollow hydroxyapatite microspheres with favorable biocompatibility while loaded with simvastatin in order to constitute sustained-release system in vitro and examine the effect in the restoration of rat dental pulp. Methods: Based on the principle of biomimetic synthesis, hollow hydroxyapatite (HHAp) microspheres were successfully synthesized via the precipitation reaction utilizing glutamic acid (Glu) and sodium dodecyl sulfonate (SDS) “core-shell model” aggregates acting as templates. Morphology of the resultant was characterized and then loaded with simvastatin. Release in vitro of simvastatin was analyzed by UV spectrophotometry and then the cumulative rate curves of simvastatin were drawn. Thirty male Wistar rats were divided into three groups randomly and established the model of direct dental pulp capping of maxillary first molars. The drug loaded microspheres containing 10^-5mol/L, 10^-7mol/L and 10^-9mol/L simvastatin were applied on the maxillary first molars on left side of the three groups respectively. The exposed pulps of the maxillary first molars on right of the first group were capped with HHAp microspheres without loading drug. The rest accepted no special treatment. On day 7 and 28 after operation, five rats of each group were sacrificed and stained with hematoxylin-eosin. The area rates of reparative dentin were calculated with NIH image software and analyzed by Dunnett t test and paired t test with SPSS 17.0 software. Results: The as-prepared HAp was hollow microspheres with diameter of 2-4 μm and was composed of many needle-like particles. The drug loading and encapsulation efficiency was 21.3%-46.0% and 34.46%-46.02%, respectively. The drug-loading release behavior in vitro followed the linear release. The inflammation under the perforation in group control was more obvious than experimental groups at day 7. A thicker layer of reparative dentin was formed in group 10^-7mol/L than group control, 10^-5mol/L and 10^-9mol/L. Conclusion: The HHAp microspheres can be synthesized via Glu and SDS “core-shell model” aggregates acting as templates and the efficiency of drug loading and encapsulation is high. The release behavior follows the linear release. The drug loaded microspheres containing 10^-7mol/L SIM may promote the repair of dental pulp after injury.

Key words: Hollow hydroxyapatite , Simvastatin , Sustained release, Dental pulp , Wound healing

CLC Number:

R780.1

WANG Ke, ZHAO Xu, LI Yi, ZHANG Xue, WANG Yin-jing.. Preparation and Characterization of Hollow Hydroxyapatite Microspheres Loaded with Simvastatin as Pulp Capping Agent.[J]. Journal of Oral Science Research, 2016, 32(7): 707-711.

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