Anti-cariogenic and Anti-biofilm Effect of Asiatic Acid on Streptococcus Mutans

doi:10.13701/j.cnki.kqyxyj.2021.05.006

Abstract

Abstract: Objective: To analyze the inhibitory effect of asiatic acid on Streptococcus mutans (S.mutans) biofim formation and its acid and extracellular polysaccharides (EPS) production. Methods: The planktonic bacterial susceptibility assay was used to determine the minimum inhibitory concentration (MIC) of asiatic acid on S.mutans. Crystal violet assay and MTT assay were performed to determine the effect of asiatic acid on the biomass and metabolic activity of S.mutans biofilm. Anthrone-sulfuric acid method, field emission scanning electron microscopy (FESEM), and confocal laser scanning microscopy (CLSM) were performed to observe the biofilm morphology and EPS synthesis after treatment by asiatic acid. The effect of asiatic acid on the acid production of S. mutans was performed by pH drop assay. CCK-8 kit assay was conducted to assess the cytotoxicity of asiatic acid. Results: The MIC of asiatic acid on S. mutans was 20 μg/mL. The sub-MICs (10 μg/mL and 5 μg/mL) were all effective on the reduction of the biofilm formation, acid production and EPS synthesis of S. mutans. No significant cytotoxicity of asiatic acid was found. Conclusion: Asiatic acid is effective on the inhibition of S.mutans biofilm formation, its acid production, and EPS synthesis

Key words: asiatic acid, Streptococcus mutans, biofilm

SHUI Yusen, YANG Ran. Anti-cariogenic and Anti-biofilm Effect of Asiatic Acid on Streptococcus Mutans[J]. Journal of Oral Science Research, 2021, 37(5): 401-406.

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